The study was conducted at the University of Education Winneba, Mampong-Campus (forest-transition) in the Ashanti Region of Ghana to examine the different rates of nitrogen fertilizer on stem borer infestation in three varieties of maize. The treatments were three maize varieties, Obatanpa (V1), Mamaba (V2), Golden Jubilee (V3) and four levels of nitrogen,0kgN/ha (N0), 45kgN/ha (N1), 60kgN/ha (N2), 90 kgN/ha (N3). The experimental design used was 3×4 factorial trial fitted into randomized complete block design (RCBD) with 12 treatment combinations and 4 replications. Factor A was designated as the maize varieties and factor B as the rates of nitrogen fertilizer. The results of the study indicated that higher nitrogen application leads to higher stem borer infestation and damage with the number of stem borers fluctuating significantly at weekly intervals during the minor and the major cropping seasons.