Occurrence of Colistin Resistance in Poultry Production in Wa: a Case Study of Lamdi Farms And Trading Enterprise

Authors

  • Osisiogu Emmanuel Dr. Hilla Limann Technical University
  • Henry Appiahkorang Department of Science Laboratory Technology, Dr. Hilla Limann Technical University, Wa, Ghana
  • Mahama Francis Bangya Department of Science Laboratory Technology, Dr. Hilla Limann Technical University, Wa, Ghana
  • kinsley Duah Department of Science Laboratory Technology, Dr. Hilla Limann Technical University, Wa, Ghana
  • Helen Chioma Udeorah Department of Science Laboratory Technology, Dr. Hilla Limann Technical University, Wa, Ghana
  • Flavia Kaduni Bawa West African Centre for Cell Biology of Infectious Pathogens, (WACCBIP) Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Ghana
  • Andrews Kwabena Sah Laboratory Department, Bogoso Government Hospital, Bogoso, Ghana
  • Frederick Allou Kwame Nkrumah University of Science and Technology, Department of Clinical Microbiology, Kumasi-Ghana.

Keywords:

Multidrug Resistance, Colistin-Resistant Bacteria, Antimicrobial Resistance, Poultry, Colistin, Chicken, MCR gene

Abstract

This study aimed to determine the prevalence of phenotypic colistin resistance among bacteria isolated from poultry production settings in Wa, Ghana. Fecal samples were collected from adult birds, young birds, and import boxes at farms in the Wa Municipality. Bacteria were isolated and identified using standard biochemical testing. The most common organisms identified were Escherichia coli (27%) and Citrobacter species (27%). The Kirby-Bauer disc diffusion and broth microdilution minimum inhibitory concentration (MIC) methods were used for antimicrobial susceptibility testing against colistin. By MIC testing, 7 isolates (50%) displayed colistin resistance, as against only 2 isolates (14%) by Kirby-Bauer. This discrepancy between test methods reinforces literature indicating poorer sensitivity of disc diffusion and MIC for resistance detection. Resistance rates were highest in import box isolates (75% by MIC), followed by young birds (60%), and lowest in adult birds (20%). Alarming levels of colistin resistance were detected, particularly among bacteria from import boxes. This suggests likely transmission via international poultry trade. Prudent antibiotic use and infection control are critical to preventing further emergence and spread of colistin resistance in poultry production in Ghana. Expanded monitoring, along with molecular and genomic analysis, are warranted to fully characterize resistance mechanisms and epidemiology using the reliable MIC methodology over disc diffusion for accurate resistance detection. Overall, proactive measures are urgently required to combat the rising threat of colistin resistance globally.

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Published

2023-12-31

How to Cite

Emmanuel, O., Appiahkorang , H., Bangya, M. F. ., Duah , kinsley, Udeorah, H. C., Bawa, F. K. ., Sah, A. K., & Allou, F. (2023). Occurrence of Colistin Resistance in Poultry Production in Wa: a Case Study of Lamdi Farms And Trading Enterprise. ADRRI Journal of Medical and Biomedical Research, 5(1(6) October-December), 1-15. Retrieved from https://journals.adrri.org/index.php/adrrijmbr/article/view/1056