This study focuses on the awareness, access, purpose and frequency of use of electronic resources and the challenges confronting male and female users in accessing electronic resources in the library of Valley View University, Ghana. The descriptive survey method was the design adopted for this study. Questionnaires were the main instrument for data collection. The population of the students was 336 which comprised 188 males and 148 female students from the School of Business – Accounting option. Statistical Package for Social Sciences (SPSS 16 Version) was used to analyse the data. The study disclosed that the gap between male and female students on the use of e-resources at VVU is quite negligible.